Banana somaclonal variation assessed by Amplified Fragment Length Polymorphism profiles at early cycles of in vitro culture
نویسندگان
چکیده
Banana micropropagation for obtaining free-virus plants frequently provokes somaclonal variation that could increase useful genetic variability in this asexually propagated crop. Both exploring the cycle of vitro culture which occurs and amount generated polymorphism, are necessary. In work, preliminary results during early cycles banana reported. Four randomly selected regenerated from fifth two samples mother plant were analyzed. A total 36 AFLP primer combinations assayed, 24 them produced amplicons varying among 50- 500 bp. The presented a 125 different while jointly showed 131 with mean 119.75 ± 3.97 per individual. High level DNA polymorphism (24.43 %) was found micropropagated and, additionally, occurrence at earlier suggested by multivariate analysis Principal Coordinates. study, validated adequacy technique to assess it molecular verified. phenotypic effects detected variations remain be evaluated.
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ژورنال
عنوان ژورنال: Agriscientia
سال: 2021
ISSN: ['1668-298X', '0327-6244']
DOI: https://doi.org/10.31047/1668.298x.v38.n2.33260